gnotobiotic Syllabification: (gno·to·bi·ot·ic)Pronunciation: /ˌnōtōbīˈätik/Definition of gnotobiotic adjective Biology of, relating to, or denoting an environment for rearing or culturing organisms in which all the microorganisms are either known or excluded. Origin: 1940s: from Greek gnōtos 'known' + bioticgnotobiotic in other Oxford dictionaries Definition of gnotobiotic in the British & World English dictionary