endonuclease Syllabification: (en·do·nu·cle·ase)Pronunciation: /ˌendōˈn(y)o͞oklēˌās, -ˌāz/Definition of endonuclease noun Biochemistry an enzyme that cleaves a polynucleotide chain by separating nucleotides other than the two end ones. endonuclease in other Oxford dictionaries Definition of endonuclease in the British & World English dictionary