chromatid Syllabification: (chro·ma·tid)Pronunciation: /ˈkrōməˌtid/Definition of chromatid noun Biology each of the two threadlike strands into which a chromosome divides longitudinally during cell division. Each contains a double helix of DNA. Origin: early 20th century: from Greek khrōma, khrōmat- 'color' + -id2chromatid in other Oxford dictionaries Definition of chromatid in the British & World English dictionary