acidophilus Syllabification: (ac·i·doph·i·lus)Pronunciation: /ˌasiˈdäfələs/Definition of acidophilus noun a bacterium that is used to make yogurt and to supplement the intestinal flora. Lactobacillus acidophilus, a Gram-positive rod-shaped bacterium Origin: 1920s: modern Latin, literally 'acid-loving'acidophilus in other Oxford dictionaries Definition of acidophilus in the British & World English dictionary