acetylcholinesterase Syllabification: (a·ce·tyl·cho·lin·es·ter·ase)Pronunciation: /əˌsētlˌkōləˈnestəˌrās, -ˌrāz, ˌasitl-/Definition of acetylcholinesterase noun Biochemistry an enzyme that causes rapid hydrolysis of acetylcholine. Its action serves to stop excitation of a nerve after transmission of an impulse. acetylcholinesterase in other Oxford dictionaries Definition of acetylcholinesterase in the British & World English dictionary