acetylcholine Syllabification: (a·ce·tyl·cho·line)Pronunciation: /əˌsētlˈkōˌlēn, ˌasitl-/Definition of acetylcholine noun Biochemistry a compound that occurs throughout the nervous system, in which it functions as a neurotransmitter. acetylcholine in other Oxford dictionaries Definition of acetylcholine in the British & World English dictionary