topoisomerasePronunciation: /ˌtɒpəʊˈʌɪsəməreɪz/Definition of topoisomerase noun Biochemistry an enzyme which alters the supercoiled form of a DNA molecule. Origin: 1970s: from Greek topos 'place' + isomer + -asetopoisomerase in other Oxford dictionaries Definition of topoisomerase in the US English dictionary