tetracyclinePronunciation: /ˌtɛtrəˈsʌɪkliːn/Definition of tetracycline noun Medicine any of a large group of antibiotics with a molecular structure containing four rings. These antibiotics are often obtained from bacteria of the genus Streptomyces Origin: 1950s: from tetra- + cyclic + -ine4tetracycline in other Oxford dictionaries Definition of tetracycline in the US English dictionary