speciationPronunciation: /ˌspiːʃɪˈeɪʃ(ə)n, ˌspiːs-/Definition of speciation noun [mass noun] Biology the formation of new and distinct species in the course of evolution. Derivatives speciatePronunciation: /ˈspiː-/ verb speciation in other Oxford dictionaries Definition of speciation in the US English dictionary