sonicatePronunciation: /ˈsɒnɪkeɪt/ BiochemistryDefinition of sonicate verb [with object] subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes: cells were sonicated in an ice bath noun a biological sample which has been sonicated. Derivatives sonication noun Origin: 1950s: from sonic + -ate2sonicate in other Oxford dictionaries Definition of sonicate in the US English dictionary