pyrimidinePronunciation: /pɪˈrɪmɪdiːn/Definition of pyrimidine noun [mass noun] Chemistry a colourless crystalline compound with basic properties. A heteroaromatic compound; chemical formula: C4H4N2 (also pyrimidine base) [count noun] a substituted derivative of pyrimidine, especially the bases thymine and cytosine present in DNA. Origin: late 19th century: from German Pyrimidin, from pyridine, with the insertion of -im- from imidepyrimidine in other Oxford dictionaries Definition of pyrimidine in the US English dictionary