methicillinPronunciation: /ˌmɛθɪˈsɪlɪn/Definition of methicillin noun [mass noun] Medicine a semi-synthetic form of penicillin used against staphylococci which produce penicillinase. Origin: 1960s: from meth(yl) and (pen)icillinmethicillin in other Oxford dictionaries Definition of methicillin in the US English dictionary