iotaPronunciation: /ʌɪˈəʊtə/Translate iota | into German | into Italian | into Spanish Definition of iota noun 1the ninth letter of the Greek alphabet (Ι, ι), transliterated as ‘i’. (Iota) [followed by Latin genitive] Astronomy the ninth star in a constellation:Iota Piscium 2 [in singular, usually with negative] an extremely small amount:nothing she said seemed to make an iota of differenceOrigin:from Greek iōta. sense 2 arose because iota is the smallest letter of the Greek alphabet: compare with jotiota in other Oxford dictionaries Definition of iota in the US English dictionary