guaninePronunciation: /ˈgwɑːniːn/Definition of guanine noun [mass noun] Biochemistry a compound that occurs in guano and fish scales, and is one of the four constituent bases of nucleic acids. A pure derivative, it is paired with cytosine in double-stranded DNA. Alternative name: 6-oxy-2-aminopurine; chemical formula: C5H5N5O Origin: mid 19th century: from guano + -ine4guanine in other Oxford dictionaries Definition of guanine in the US English dictionary