acidophilusPronunciation: /ˌasɪˈdɒfɪləs/Definition of acidophilus noun [mass noun] a bacterium that is used to make yogurt and to supplement the intestinal flora. Lactobacillus acidophilus; a Gram-positive rod-shaped bacterium Origin: 1920s: modern Latin, literally 'acid-loving'acidophilus in other Oxford dictionaries Definition of acidophilus in the US English dictionary