acetylcholinesterasePronunciation: /ˌasɪtʌɪlˌkəʊlɪnˈɛstəreɪz, -tɪl-/Definition of acetylcholinesterase noun [mass noun] Biochemistry an enzyme that causes rapid hydrolysis of acetylcholine. Its action serves to stop excitation of a nerve after transmission of an impulse. acetylcholinesterase in other Oxford dictionaries Definition of acetylcholinesterase in the US English dictionary