subfamily Syllabification: (sub·fam·i·ly)Pronunciation: /ˈsəbˌfam(ə)lē/Definition of subfamily noun (plural subfamilies) a subdivision of a group. Biology a taxonomic category that ranks below family and above tribe or genus, usually ending in -inae (in zoology) or -oideae (in botany). subfamily in other Oxford dictionaries Definition of subfamily in the British & World English dictionary