phosphorylate Syllabification: (phos·pho·ryl·ate)Pronunciation: /ˈfäsfərəˌlāt, fäsˈfôrə-/Definition of phosphorylate verb [with object] chiefly Biochemistry introduce a phosphate group into (a molecule or compound). Derivatives phosphorylationPronunciation: /ˈfäsfərəˈlāSHən/ noun phosphorylate in other Oxford dictionaries Definition of phosphorylate in the British & World English dictionary