nucleotide Syllabification: (nu·cle·o·tide)Pronunciation: /ˈn(y)o͞oklēəˌtīd/Definition of nucleotide noun Biochemistry a compound consisting of a nucleoside linked to a phosphate group. Nucleotides form the basic structural unit of nucleic acids such as DNA. nucleotide in other Oxford dictionaries Definition of nucleotide in the British & World English dictionary