nisin Syllabification: (ni·sin)Pronunciation: /ˈnīsin/Definition of nisin noun an antibiotic substance that is a mixture of related polypeptides and is used in some countries as a food preservative. This substance is produced by the bacterium Streptococcus lactis Origin: 1940s: from (Group) N i(nhibitory) s(ubstance) + -in1nisin in other Oxford dictionaries Definition of nisin in the British & World English dictionary