exonuclease Syllabification: (ex·o·nu·cle·ase)Pronunciation: /ˌeksōˈn(y)o͞oklēˌās/Definition of exonuclease noun Biochemistry an enzyme that removes successive nucleotides from the end of a polynucleotide molecule. exonuclease in other Oxford dictionaries Definition of exonuclease in the British & World English dictionary