cytosine Syllabification: (cy·to·sine)Pronunciation: /ˈsītəˌsēn/Definition of cytosine noun Biochemistry a compound found in living tissue as a constituent base of nucleic acids. It is paired with guanine in double-stranded DNA. A pyrimidine derivative; chemical formula: C4H5N3O cytosine in other Oxford dictionaries Definition of cytosine in the British & World English dictionary