counterstain Syllabification: (coun·ter·stain)Pronunciation: /ˈkountərˌstān/ BiologyDefinition of counterstain noun an additional dye used in a microscopy specimen to produce a contrasting background or to make clearer the distinction between different kinds of tissue. verb [with object] treat (a specimen) with a counterstain. counterstain in other Oxford dictionaries Definition of counterstain in the British & World English dictionary