Rio Negro Syllabification: (Ri·o Ne·gro)Pronunciation: /ˈrē-ō ˈnāgrō/Definition of Rio Negro a river in South America that rises as the Guainia in eastern Colombia and flows for about 1,400 miles (2,255 km) through northwestern Brazil before joining the Amazon River near Manaus. Rio Negro in other Oxford dictionaries Definition of Rio Negro in the British & World English dictionary